A simple, efficient method for the separation of humic substances and DNA from environmental samples.
نویسندگان
چکیده
Three different gels (Sepharose 4B, Sephadex G-200, and Sephadex G-50) were evaluated as a means of removing humic contaminants from DNA extracts of environmental samples. Sepharose 4B gave superior separation of DNA from humics, and DNA purified in this way showed consistently greater amplification than DNA purified by the other materials.
منابع مشابه
The simultaneous removal of turbidity and humic substances from water using the enhanced coagulation process
This study aimed to investigate the efficiency of the enhanced coagulation (EC) process for the simultaneous removal of turbidity and humic substances (HS) from raw water from the Sanandaj Water Treatment Plant (SWTP). This study was conducted on a laboratory scale using a jar test device and ferric chloride (FeCL3) as the coagulant. Accordingly, the effects of pH and coagulant dosag...
متن کاملبررسی کارائی نانوذرات سیلیکون در جذب اسیدهیومیک موجود در آب
Background and Objective: Humic substances in drinking water react with chlorine and form carcinogenic compounds. Humic acid is a principal component of humic substances and its separation from surface waters is crucial. Adsorption process is among different methods for separation of humic acid from surface waters and because of its simple and economical nature it has attracted considerable att...
متن کاملApplication of experimental design approach for optimization of the photocatalytic degradation of humic substances in aqueous solution using immobilized ZnO nanoparticles
Degradation of humic substances in water is important due to its adverse effects on the environment and human health. The aim of this study was modeling and investigating the degradation of humic substances in water using immobilized ZnO as a catalyst. ZnO nanoparticles were synthesized through simple coprecipitation (CPT) method and immobilized on glass plates. The immobilized ZnO nanocatalyst...
متن کاملStudying the Efficiency of XAD-7HP Anionic Resin in the Extraction of Humic Acid from Surface Water
Introduction: Humic substances (HSs) are very complex and heterogeneous organic matter with high molecular weight and various functional groups, which are produced from the chemical and biological degradation of plants and animal tissues. HS can cause: colour, odour and taste problems in drinking water, biological growth in distribution systems, increase in chlorine demand for disinfection, mem...
متن کاملRapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction.
The polymerase chain reaction (PCR) was used to amplify an Escherichia coli 16S ribosomal gene fragment from sediments with high contents of humic substances. Total DNA was extracted from 1 g of E. coli seeded or unseeded samples by a rapid freeze-and-thaw method. Several approaches (use of Bio-Gel P-6 and P-30 and Sephadex G-50 and G-200 columns, as well as use of the Stoffel fragment) were us...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied and environmental microbiology
دوره 63 12 شماره
صفحات -
تاریخ انتشار 1997